Journal: OncoTargets and Therapy

Article Title: Exosomal microRNA-141 is upregulated in the serum of prostate cancer patients

doi: 10.2147/ott.s95565

Figure Lengend Snippet: Figure 1 Characterization of isolated serum exosomes. Notes: (A) Exosomes isolated from serum samples were homogeneous in appearance under transmission electron microscopy (scale bars, 5 µm in left panel and 100 nm in right panel). (B) The expression levels of HSP70 and CD63 were detected in exosomes isolated from serum samples by Western blot analysis (lane 1, serum exosomal pellets; lane 2, exosomes-depleted serum supernatant). β-Actin was used as loading control. (C) The expression of CD63 was detected in exosomes isolated from serum samples by flow cytometry analysis. Abbreviations: HSP70, heat shock protein 70; PE, phycoerythrin.

Article Snippet: Subsequently, the membranes were blocked for 1 hour at room temperature with blocking buffer (LI-COR Biosciences, Lincoln, NE, USA), and incubated overnight at 4°C with rabbit antihuman heat shock protein 70 antibody and CD63 antibody (1:1,000; RayBiotech, Norcross, GA, USA). β-Actin (1:1,000; Abcam, Cambridge, MA, USA) was used as loading control.

Techniques: Isolation, Transmission Assay, Electron Microscopy, Expressing, Western Blot, Control, Flow Cytometry